The N-terminal NH 2- and C-terminal COOH-group of a protein also influences the charge of a polypeptide 21, 22, 23, 24, 25. The dissociation constant ( pK a) of a polypeptide is determined by the presence of seven different charged amino acids arginine, aspartate, cysteine, glutamate, histidine, tyrosine, and lysine 18, 19, 20. The pI indicates the pH at which the net charge of a protein is zero 1. Plant cells contain a vast array of proteins with different molecular weight and pI 1. The shape, size, solubility, and pI of a protein determine its ability to move across different cellular compartments and also determine their function 12, 13, 14, 15, 16, 17. Post-translational modifications that occur can alter the function of a protein and contribute to the ability to target the location of a protein to a specific subcellular compartment 9, 10, 11. These proteins have diverse molecular weights and isoelectric points ( pIs) 1, 2. The total mRNA of an organism encodes a wide array of proteins that vary in cellular function and homeostasis 6, 7, 8. Proteomes comprise all of the translated products of nucleotide sequences contained in messenger RNA (mRNA) 3, 4, 5. Further, the molecular weight and isoelectric point of individual proteins can enable us to understand their functional significance in different species.Įach and every molecule in a cell has its own special characteristics, including the individual proteins that comprise the proteome of an organism 1, 2. The individual 2D virtual proteome map of the plant kingdom will enable us to understand the proteome diversity between different species. The database can be found in the following link. The PlantMWpIDB database also allows one to query protein sequences. ![]() The database represents a virtual 2D proteome map of plants, and the molecular weight and pI of a protein can be obtained by searching on the name of a protein, a keyword, or by a list of accession numbers. PlantMWpIDB provides an easy-to-use and efficient interface for search options and generates a summary of basic protein parameters. The Molecular weight and isoelectric point ( pI) are important molecular parameters of proteins that are useful when conducting protein studies involving 2D gel electrophoresis, liquid chromatography-mass spectrometry, and X-ray protein crystallography. A database, PlantMWpIDB, containing protein data from 342 plant proteomes was created to provide information on plant proteomes for hypothesis formulation in basic research and for biotechnological applications. Therefore, we have calculated the molecular weight and isoelectric point of the plant proteins and reported them in this database. Although the genome sequence data of the plant kingdom improved enormously, the proteomic details have been poorly elaborated. ![]() The molecular weight and isoelectric point of the proteins are very important parameters that control their subcellular localization and subsequent function.
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